detection of avian leukosis virus (alv) in albumen of shiraz commercial and local layer flocks using elisa and rt-pcr
نویسندگان
چکیده
avian leukosis viruses (alvs) cause different types of tumours in poultry and can affect the health and egg production of the birds. to investigate the presence of the virus in chicken layer flocks in shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested by antigen-capture enzyme-linked immunosorbent assay (ac-elisa). the results showed that 3.33, 76 and 80% of commercial broiler breeder, local layer breeder and local layer grand parent were positive, respectively. thirty-five albumen samples were randomly selected and tested by rt-pcr using pu1/pu2 and pa1/pa2 primer sets. the samples with elisa s/p ratio equal or more than 0.17 were positive by rt-pcr using pa1/pa2 primers. this is the first report of the presence of the alv in egg albumen samples of chicken layer flocks in shiraz.
منابع مشابه
Detection of avian leukosis virus (ALV) in albumen of Shiraz commercial and local layer flocks using ELISA and RT-PCR
Avian leukosis viruses (ALVS) cause different types of tumours in poultry and can affect the health and egg production of the birds. To investigate the presence of the virus in chicken layer flocks in Shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...
متن کاملDetection of avian leukosis virus subgroup J in albumen of commercial and native fowl eggs using RT-PCR in Fars province of Iran
The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since1989. This virus is responsible for economic losses due to both mortality and depressed performance inchickens. So, the objective of this study is the detection of ALV-J in the albumen of commercial and nativefowl eggs using RT-PCR. Three hundred and seventy egg albumens were randomly selected from dif...
متن کاملdetection of avian leukosis virus subgroup j in albumen of commercial and native fowl eggs using rt-pcr in fars province of iran
the subgroup j of alv (alv-j) has emerged as an important pathogen of meat-type chickens since1989. this virus is responsible for economic losses due to both mortality and depressed performance inchickens. so, the objective of this study is the detection of alv-j in the albumen of commercial and nativefowl eggs using rt-pcr. three hundred and seventy egg albumens were randomly selected from dif...
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عنوان ژورنال:
iranian journal of veterinary researchناشر: shiraz university
ISSN 1728-1997
دوره 9
شماره 3 2008
میزبانی شده توسط پلتفرم ابری doprax.com
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